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Category: Biochemistry

Experiments under the department of Biochemistry

Determination of Percentage of Water in a Hydrate

Determination of Percentage of Water in a Hydrate

Introduction      Hydrates are compounds containing water molecules combined in a definite ratio as an integral part of the crystal. These hydrates are also said to contain water of crystallization or water of hydration. Each hydrate has a definite composition; a certain number of water molecules are combined with each formula unit of the salt. Examples of hydrates are: CuSO4•5H2O Copper(II) sulfate pentahydrate CoCl2 â€¢ 6H2O Cobalt (II) chloride hexahydrate ZnSO4 â€¢ 7H2O Zinc sulfate heptahydrate Objective of the Experiment This experiment is…

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Determination of Heat of Reaction from the Temperature Dependence of the Equilibrium Constant

Determination of Heat of Reaction from the Temperature Dependence of the Equilibrium Constant

Introduction The basis of this experiment is to determine the heat of reaction from the temperature dependence of equilibrium constant. The reaction basically involves dissolving borax Na2B4O7.10H2O in water. Borax is sparingly soluble in water and its solubility properties is calculated at different temperature .Quantities like enthalpy ∆H0, the entropy ∆S0, free energy ∆G0 as well as Ksp are determined for the reaction system. Saturated solution of borax are to be obtained, then it is subjected to further analysis. This makes the…

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Determination of Heat of Neutralization

Determination of Heat of Neutralization

Introduction      Thermochemistry deals with the experimental measurement and calculation of heat changes.Every chemical reaction is always accompanied by a change in energy usually in form of heat: a reaction will either give off heat (exothermic), or it will absorb heat (endothermic). Many times the heat transfer associated with a chemical reaction is more important than the reaction itself. The heat change that accompanies the reaction of one mole of substance is designated by the symbol Q. Heat changes for chemical…

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Safety Guidelines in the Laboratory

Safety Guidelines in the Laboratory

Safety goggles, protective wears, gloves, shoes, helmet, and other Personal Protective Equipments (PPEs) should be used when necessary especially when working with hazardous chemicals Only materials needed for the day’s laboratory exercise should be placed on bench work Always familiarize yourself in advance with an experiment before going to the lab No eating, drinking or any other mouth activity while in the lab Personal materials like bags, books, phones should be kept away from work bench to avoid damage or…

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